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Western blot analysis of CX3CR1 in THP-1 cell lysate with CX3CR1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of CX3CR1 in human spleen cells with CX3CR1 antibody at 10 μg/mL.
Immunofluorescence of CX3CR1 in THP-1 cells with CX3CR1 antibody at 20 μg/mL.
Flow Cytometry Validation of CX3CR1 in THP-1 Cells 1X10^6/mL THP-1 cells were intracellularly stained with 2 μg/mL of CX3CR1 antibody (red) or Rabbit IgG isotype control (black) at RT for 30 min, followed by PSFlo 488 conjugated goat anti-rabbit IgG secondary antibody staining (1:1000) at RT for 30 min. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100 in PBS.
Western blot analysis of CX3CR1 in THP-1 cell lysate with CX3CR1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

CX3CR1 Antibody

CATALOG NUMBER: 2201

Clonality:
Polyclonal
Tested Applications:
ELISA, FC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Rabbit: (93%), Mouse: (93%), Bovine: (86%)
Immunogen:
CX3CR1 antibody was raised against a peptide corresponding to amino acids in a extracellular loop of human CX3CR1.
The immunogen is located within amino acids 160 - 210 of CX3CR1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF, IHC-P, WB
Application Note:
WB: 0.5-1 μg/mL; IHC-P: 2-10 μg/mL; IF: 20 μg/mL; FC: 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry, Immunofluorescence and Flow Cytometry in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of CX3CR1 are known to exist.
Positive Control 1:
Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 2:
Cat. No. 1212 - 3T3 Cell Lysate
Positive Control 3:
Cat. No. 17-008 - THP-1 Cell Slide
Predicted Molecular Weight:
Predicted: 39, 43 kDa
Observed: 50 kDa
Purification:
CX3CR1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CX3CR1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CX3CR1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CX3CR1
Additional Names:
CX3CR1 Antibody: V28, CCRL1, GPR13, CMKDR1, GPRV28, CMKBRL1, CX3C chemokine receptor 1, Beta chemokine receptor-like 1, C-X3-C CKR-1
Protein Accession Number:
NP_001328
Protein Gi Number:
4503171
Ncbi Gene Id Number:
1524
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CX3CR1 Antibody: CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was initially identified as the receptor for the transmembrane molecule fractalkine, and renamed CX3CR1. CX3CR1 is known to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.
Background Reference 1:
Raport CJ, Schweickart VL, Eddy RL JR, et al. The orphan G-protein-coupled receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues. Gene 1995; 163:295-9
Background Reference 2:
Combadiere C, Ahuja SK , and Murphy PM. Cloning, chromosomal localization, and RNA expression of a human β chemokine receptor-like gene. DNA Cell Biol. 1995;14:673-80
Background Reference 3:
Combadiere C, Gao J, Tiffany HL, et al. Gene cloning, RNA distribution, and functional expression of mCX3CR1, a mouse chemotactic receptor for the CX3C chemokine fractalkine. Biochem. Biophys. Res. Commun. 1998; 253:728-32
Background Reference 4:
Harrison JK, Barber CM, and Lynch KR. cDNA cloning of a G-protein-coupled receptor expressed in rat spinal cord and brain related to chemokine receptors. Neurosci. Lett. 1994; 169:85-9

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2201

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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