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Western blot analysis of lysates from A549 cells using CXCL10/IP-10 Rabbit mAb at 1:9000 dilution incubated overnight at 4℃. A549 cells were treated with TNF-α (20ng/mL) for 6 hours, Brefeldin A (300ng/mL) for 3 hours and IFN-γ (50ng/mL) for 8 hours of stimulation. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.
Western blot analysis of lysates from HT-29 cells using CXCL10/IP-10 Rabbit mAb at 1:9000 dilution incubated overnight at 4℃. HT-29 cells were treated with TNF-α (20ng/mL) for 6 hours, Brefeldin A (300ng/mL) for 3 hours and IFN-γ (50ng/mL) for 8 hours of stimulation. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.
Western blot analysis of lysates from THP-1 cells using CXCL10/IP-10 Rabbit mAb at 1:9000 dilution incubated overnight at 4℃. THP-1 cells were treated with LPS (1 μg/mL) for 6 hours, Brefeldin A (300ng/mL) for 3 hours and IFN-γ (50ng/mL) for 8 hours of stimulation. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.
Western blot analysis of lysates from A549 cells using CXCL10/IP-10 Rabbit mAb at 1:9000 dilution incubated overnight at 4℃. A549 cells were treated with TNF-α (20ng/mL) for 6 hours, Brefeldin A (300ng/mL) for 3 hours and IFN-γ (50ng/mL) for 8 hours of stimulation. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.

CXCL10/IP-10 Rabbit Recombinant Antibody (83-537)

CATALOG NUMBER: 83-537

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:9000 - 1:54000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
A549 cells and HT-29 cells were treated with TNF-α, Brefeldin A and IFN-γ、THP-1 cells were treated with LPS, Brefeldin A and IFN-γ.
Predicted Molecular Weight:
11kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CXCL10
Additional Names:
C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10; CXCL10/IP-10
Protein Accession Number:
P02778
Ncbi Gene Id Number:
3627
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. This gene may also be a key regulator of the 'cytokine storm' immune response to SARS-CoV-2 infection.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-537

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

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