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Antibody used in WB on Human Lung at 0.2-1 ug/ml.
Antibody used in WB on Human Lung at 0.2-1 ug/ml.

CXCL12 Antibody

CATALOG NUMBER: 31-056

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CXCL12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CXCL12 antibody can be used for detection of CXCL12 by ELISA at 1:62500. CXCL12 antibody can be used for detection of CXCL12 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10410 - Fetal Lung Tissue Lysate
Predicted Molecular Weight:
11 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CXCL12 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CXCL12
Additional Names:
CXCL12, IRH, PBSF, SDF1, TLSF, TPAR1, SCYB12
Protein Accession Number:
NP_000600
Protein Gi Number:
10834988
Ncbi Gene Id Number:
6387
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF, or IL1. The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group.
Background Reference 1:
Sadir, R., et al., (2004) J. Biol. Chem. 279 (42), 43854-43860.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-056

List Size:
100 ul

List Price:

$519.00

Datasheet
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