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Western blot analysis of CXCL12 in HeLa cell lysate with CXCL12 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of CXCL12 in HeLa cells with CXCL12 antibody at 5 μg/mL.
Western blot analysis of CXCL12 in HeLa cell lysate with CXCL12 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

CXCL12 Antibody

CATALOG NUMBER: 7591

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
CXCL12 antibody was raised against a 16 amino acid peptide near the center of human CXCL12 .
The immunogen is located within the last 50 amino acids of CXCL12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
CXCL12 antibody can be used for detection of CXCL12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
CXCL12 antibody is human and mouse reactive. Multiple isoforms of CXCL12 are known to exist.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 15 kDa
Observed: 18 kDa
Purification:
CXCL12 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CXCL12 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CXCL12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CXCL12
Additional Names:
CXCL12 Antibody: IRH, PBSF, SDF1, TLSF, TPAR1, SCYB12, SDF1A, SDF1B, Stromal cell-derived factor 1, C-X-C motif chemokine 12, SDF-1
Protein Accession Number:
NP_001171605
Protein Gi Number:
296011023
Ncbi Gene Id Number:
6387
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The CXCL12 protein, also known as SDF1, is a stromal cell-derived alpha chemokine member of the intercrine family. CXCL12 functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4 (CXCR4) and CXCR7, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis (reviewed in 1). Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections (2).
Background Reference 1:
Timotijevic G, Mostarica Stojkovic M, and Miljkovic D. CXCL12: role in neuroinflammation. Int. J. Biochem. Cell Biol. 2012; 44:838-41.
Background Reference 2:
Reiche EM, Ehara Watanabe MA, Bonametti AM, et al. The effect of stromal cell-derived factor 1 (SDF1/CXCL12) genetic polymorphism on HIV-1 disease progression. Int. J. Mol. Med. 2006; 18:785-93.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7591

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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