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Western blot analysis of extracts of various cell lines, using CXCR1 antibody (16-582) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat lung using CXCR1 antibody (16-582) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using CXCR1 antibody (16-582) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CXCR1 antibody (16-582) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using CXCR1 antibody (16-582) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

CXCR1 Antibody

CATALOG NUMBER: 16-582

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human CXCR1 (NP_000625.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Rat lung
Positive Control 2:
Rat spleen
Predicted Molecular Weight:
Observed: 70kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CXCR1
Additional Names:
IL8RA, interleukin 8 receptor, alpha, interleukin 8 receptor alpha, IL8RBA, IL8R1, IL-8 receptor type 1, CXCR1, CMKAR1, CKR-1, chemokine (C-X-C motif) receptor 1, CDw128a, CD181, CD128, C-C-CKR-1, C-C, IL8RA
Ncbi Gene Id Number:
3577
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-582

List Size:
100 uL

List Price:

$436.00

Datasheet
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