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42-981 (0.3ug/ml) staining of Mouse Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-981 (0.3ug/ml) staining of Mouse Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Cxcr2 Antibody

CATALOG NUMBER: 42-981

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
The immunogen for this antibody is: EFKVDKFNIED-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Mouse Colon lysates (calculated MW of 40.4kDa according to NP_034039.1). Recommended concentration: 0.3-1ug/ml.An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
Reported isoforms represent identical protein: NP_001548.1, NP_001161770.1. The immunizing peptide represent part of an extracellular domain.
Positive Control 1:
Cat. No. 1411 - Mouse Colon Tissue Lysate
Predicted Molecular Weight:
Approx 40 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Cxcr2
Additional Names:
Cxcr2, chemokine (C-X-C motif) receptor 2, CD128, CDw128, Cmkar2, Gpcr16, IL-8Rh, IL-8rb, IL8RA, Il8rb, mIL-8RH, C-X-C chemokine receptor type 2, CXC-R2, CXCR-2, GRO/MGSA receptor, IL-8 receptor alpha chain, IL-8R B, chemokine (C-X-C) receptor 2, high affinity interleukin-8 receptor B, interleukin 8 receptor, beta, interleukin-8 receptor type B
Protein Accession Number:
NP_034039.1
Protein Gi Number:
6753456
Ncbi Gene Id Number:
3579

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-981

List Size:
0.1 mg

List Price:

$469.00

Out of stock

Datasheet
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