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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis of CYB5A using rabbit polyclonal CYB5A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5A gene.
CYB5A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

CYB5A Antibody

CATALOG NUMBER: 57-429

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CYB5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the Central region of human CYB5A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
15 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CYB5A
Additional Names:
Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5
Protein Accession Number:
P00167
Protein Gi Number:
117809
Ncbi Gene Id Number:
1528
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a membrane-boundcytochrome that reduces ferric hemoglobin (methemoglobin) toferrous hemoglobin, which is required for stearyl-CoA-desaturaseactivity. Defects in this gene are a cause of type IV hereditarymethemoglobinemia. Three transcript variants encoding differentisoforms have been found for this gene.
Background Reference 1:
Gardner, A.M., et al. J. Biol. Chem. 285(31):23850-23857(2010)
Background Reference 2:
Kok, R.C., et al. J. Clin. Endocrinol. Metab. 95(3):994-999(2010)
Background Reference 3:
Sacco, J.C., et al. Pharmacogenet. Genomics 20(1):26-37(2010)
Background Reference 4:
Maghzal, G.J., et al. J. Biol. Chem. 283(18):12014-12025(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-429

List Size:
400 ul

List Price:

$495.00

Datasheet
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