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42-120 (0.1ug/ml) staining of Mouse Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
42-120 (0.1ug/ml) staining of Mouse Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Cyb5r3 Antibody

CATALOG NUMBER: 42-120

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: PNLERVGHPKERC
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in Mouse Liver lysates as well as in mouse and rat brain lysates (calculated MW of 34.1kDa according to mouse NP_084063.1). Recommended concentration: 0.1-1ug/ml.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Cyb5r3
Additional Names:
Cyb5r3, cytochrome b5 reductase 3, 0610016L08Rik, 2500002N19Rik, C85115, Dia-1, Dia1, WU:AL591952.1-001, WU:AL591952.1-002, WU:AL591952.1-003, WU:Cyb5r3, NADH-cytochrome b5 reductase, diaphorase 1, diaphorase 1 (NADH)
Protein Accession Number:
NP_084063.1
Protein Gi Number:
19745150
Ncbi Gene Id Number:
109754(mouse);25035(rat);
Background Reference 1:
Fernandez-Borja M, Janssen L, Verwoerd D, Hordijk P, Neefjes J, RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1. J. Cell. Sci. 2005 Jun 118 (Pt 12): 2661-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-120

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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