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42-439 (0.1ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-439 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-439 (0.1ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Cyb5r3 Antibody

CATALOG NUMBER: 42-439

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: CLPNLERVGHPKER
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 30kDa band observed in Mouse and Rat Lung lysates (calculated MW of 34.1kDa according to NP_084063.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1402 - Mouse Lung Tissue Lysate
Positive Control 2:
Cat. No. 1462 - Rat Lung Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Cyb5r3
Additional Names:
Cyb5r3, cytochrome b5 reductase 3, 0610016L08Rik, 2500002N19Rik, C85115, Dia-1, Dia1, WU:AL591952.1-001, WU:AL591952.1-002, WU:AL591952.1-003, WU:Cyb5r3, NADH-cytochrome b5 reductase, diaphorase 1, diaphorase 1 (NADH)
Protein Accession Number:
NP_084063.1
Protein Gi Number:
19745150
Ncbi Gene Id Number:
109754(mouse);25035(rat);

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-439

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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