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42-440 (0.01ug/ml) staining of Human Umbilical Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-440 (3.8ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-440 (0.01ug/ml) staining of Human Umbilical Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CYB5R3 Antibody

CATALOG NUMBER: 42-440

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CLPNLDHVGHPTER
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in Human Placenta, Testis and Umbilical Cord lysates (calculated MW of 38.2kDa according to NP_001165131.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Adrenal Gland shows heavy vesicular/granular staining in the cytoplasm of medular cells. Recommended concentration, 3-5ug/ml.
Specificity:
This antibody is expected to recognize all three reported isoforms (NP_000389.1; NP_001123291.1; NP_001165131.1).
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Positive Control 2:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 3:
Human Umbilical Cord Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CYB5R3
Additional Names:
CYB5R3, cytochrome b5 reductase 3, B5R, DIA1, NADH-cytochrome b5 reductase 3, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, OTTHUMP00000198574, OTTHUMP00000198665, diaphorase-1
Protein Accession Number:
NP_000389.1,NP_001123291.1,NP_001165131.1
Protein Gi Number:
4503327
Ncbi Gene Id Number:
1727

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-440

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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