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43-025 (0.1ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-025 (0.1ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Cyb5r4 Antibody

CATALOG NUMBER: 43-025

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: RCQLEKLALREKRFD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Rat Spleen lysates (calculated MW of 55kDa according to NP_596918.2 and 58.8kDa according to Q68EJ0-1). Recommended concentration: 0.1-0.3ug/ml.
Positive Control 1:
Cat. No. 1466 - Rat Spleen Tissue Lysate
Predicted Molecular Weight:
Approx 65 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Cyb5r4
Additional Names:
Cyb5r4, cytochrome b5 reductase 4, 2810034J18Rik, B5+B5R, C79736, Cb5cb5r, Ncb5or, b5/b5r, b5b5r, N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein, NADPH cytochrome B5 oxidoreductase, cb5/cb5R, cytochrome b-type NAD(P)H oxidoreductase, flavohemoprotein b5/b5R
Protein Accession Number:
NP_077157.2
Protein Gi Number:
194328781
Ncbi Gene Id Number:
266690(mouse);

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-025

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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