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Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).
CYC1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).

CYC1 Antibody

CATALOG NUMBER: 61-888

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This CYC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the C-terminal region of human CYC1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CYC1
Additional Names:
Cytochrome c1, heme protein, mitochondrial, Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, Cytochrome c-1, CYC1
Protein Accession Number:
P08574
Protein Gi Number:
311033458
Ncbi Gene Id Number:
1537
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
Background Reference 1:
Cummings, C., et al. Apoptosis 11(7):1121-1129(2006) Wen, J.J., et al. Free Radic. Biol. Med. 37(12):2072-2081(2004) An, J., et al. J. Biol. Chem. 279(18):19133-19140(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-888

List Size:
400 ul

List Price:

$495.00

Datasheet
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