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Western blot analysis of lysed extracts from MDA cells untreated or treated with Anisomycin using Cyclin B1 (phospho-Ser147).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Phospho-Ser147).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Cyclin B1 (phospho-Ser147).
Western blot analysis of lysed extracts from MDA cells untreated or treated with Anisomycin using Cyclin B1 (phospho-Ser147).

Cyclin B1 (phospho Ser147) Antibody

CATALOG NUMBER: 79-247

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Cyclin B1 (phospho-Ser147) antibody was raised against a peptide sequence around phosphorylation site of Serine 147 (A-F-S (p) -D-V) derived from Human Cyclin B1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of Cyclin B1 only when phosphorylated at serine 147.
Predicted Molecular Weight:
60 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CCNB1
Additional Names:
CCNB, VEGFR3,
Protein Accession Number:
NP_114172.1
Protein Gi Number:
14327896
Ncbi Gene Id Number:
891
Background:
The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Background Reference 1:
Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470. Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-247

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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