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IHC testing of human mantle cell lymphoma stained with Cyclin D1 antibody. Nuclear staining of tumor cells is seen.
Western blot testing of human and mouse samples with DCS-6 mAb.
FACS testing of Jurkat cells: Black=cells alone; Green=isotype control; Red=Cyclin D1 antibody PE conjugate
FACS testing of MCF-7 cells: Black=cells alone; Green=isotype control; Red=Cyclin D1 antibody PE conjugate
SDS-PAGE Analysis of Purified, BSA-Free Cyclin D1 Antibody (DCS-6). Confirmation of Integrity and Purity of the Antibody.
IHC testing of human mantle cell lymphoma stained with Cyclin D1 antibody. Nuclear staining of tumor cells is seen.

Cyclin D1 Antibody [DCS-6]

CATALOG NUMBER: 33-068

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
Human recombinant full length protein was used as the immunogen for this Cyclin D1 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CCND1
Additional Names:
G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1
Ncbi Gene Id Number:
595
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody recognizes a protein of 36kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-068

List Size:
100 ug

List Price:

$626.00

Datasheet
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