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Western blot analysis of extracts from MCF7 tissue using Cyclin D1 (Phospho-Thr286) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from MCF7 tissue using Cyclin D1 (Phospho-Thr286) Antibody. The lane on the right is treated with the antigen-specific peptide.

Cyclin D1 (phospho Thr286) Antibody

CATALOG NUMBER: 80-017

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Cyclin D1 (phospho Thr286) antibody was raised against a peptide sequence around phosphorylation site of threonine 286 (A-C-T (p)-P-T) derived from Human Cyclin D1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of Cyclin D1 only when phosphorylated at threonine 286.
Predicted Molecular Weight:
33 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CCND1
Additional Names:
BCL-1 , oncogene, CYL-1, PRAD1, PRAD1
Protein Accession Number:
NP_444284.1
Protein Gi Number:
116152
Ncbi Gene Id Number:
595
Background:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.
Background Reference 1:
Liou YC, et al. (2002) Proc Natl Acad Sci U S A 99, 1335-40
Background Reference 2:
Tagliati F, et al. (2006) Endocrinology 147, 3530-8
Background Reference 3:
Zou Y, et al. (2004) J Biol Chem 279, 27790-8

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

80-017

List Size:
0.1 mg

List Price:

$355.00

Out of stock

Datasheet
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