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Western blot analysis of extracts of various cell lines, using CYP11B2 antibody (15-536) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat adrenal gland using CYP11B2 antibody (15-536) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse adrenal gland using CYP11B2 antibody (15-536) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CYP11B2 antibody (15-536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CYP11B2 antibody (15-536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.

CYP11B2 Antibody

CATALOG NUMBER: 15-536

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human CYP11B2 (NP_000489.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
HepG2
Positive Control 2:
MCF7
Positive Control 3:
293T
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse liver
Positive Control 6:
Rat liver
Predicted Molecular Weight:
Observed: 50kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CYP11B2
Additional Names:
Cytochrome P450 11B2, mitochondrial, Aldosterone synthase, ALDOS, Aldosterone-synthesizing enzyme, CYPXIB2, Cytochrome P-450Aldo, Cytochrome P-450C18, Steroid 18-hydroxylase, CYP11B2
Ncbi Gene Id Number:
1585
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-536

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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