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Western blot analysis of anti-CYP1A1 Antibody in K562 cell line lysates (35ug/lane).
Western blot analysis of anti-CYP1A1 Antibody in mouse lung tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CYP1A1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-CYP1A1 Antibody in K562 cell line lysates (35ug/lane).

CYP1A1 Antibody

CATALOG NUMBER: 63-321

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This CYP1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-405 amino acids from the C-terminal region of human CYP1A1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CYP1A1
Additional Names:
Cytochrome P450 1A1, CYPIA1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1, CYP1A1
Protein Accession Number:
P04798
Protein Gi Number:
117139
Ncbi Gene Id Number:
1543
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CYP1A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. CYP1A1 gene has been associated with lung cancer risk.
Background Reference 1:
Delpisheh,A., Eur. J. Obstet. Gynecol. Reprod. Biol. 143 (1), 38-42 (2009) Zhuo,W., Cancer Invest. 27 (1), 86-95 (2009)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-321

List Size:
200 ul

List Price:

$495.00

Datasheet
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