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Western blot analysis of CYP20A1 using rabbit polyclonal CYP20A1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYP20A1 gene.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CYP20A1 using rabbit polyclonal CYP20A1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYP20A1 gene.

CYP20A1 Antibody

CATALOG NUMBER: 56-553

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CYP20A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the Central region of human CYP20A1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CYP20A1
Additional Names:
Cytochrome P450 20A1, 114--, CYP20A1
Protein Accession Number:
Q6UW02
Protein Gi Number:
74749375
Ncbi Gene Id Number:
57404
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the cytochrome P450superfamily of enzymes. The cytochrome P450 proteins aremonooxygenases that catalyze many reactions involved in drugmetabolism and synthesis of cholesterol, steroids and other lipids.This protein lacks one amino acid of the conserved heme bindingsite. It also lacks the conserved I-helix motif AGX(D,E)T,suggesting that its substrate may carry its own oxygen. [providedby RefSeq].
Background Reference 1:
Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
Background Reference 2:
Jiang, J.H., et al. Ai Zheng 23(6):672-677(2004)
Background Reference 3:
Nelson, D.R., et al. Pharmacogenetics 14(1):1-18(2004)
Background Reference 4:
Werck-Reichhart, D., et al. Genome Biol.(6), REVIEWS3003 (2000) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-553

List Size:
400 ul

List Price:

$495.00

Datasheet
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