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45-447 (4ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
45-447 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-447 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-447 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-447 (4ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

CYP26B1 Antibody

CATALOG NUMBER: 45-447

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-SQARSEDKDGRFH
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 85kDa band in human testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5kDa according to NP_063938.1. The 85kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Cerebellum shows staining of cell bodies in the Molecular Layer. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Small Intestine, Spleen and Testis. Recommended concentration: 3.75ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CYP26B1
Additional Names:
CYP26B1, cytochrome P450, family 26, subfamily B, polypeptide 1, CYP26A2, DKFZp686G0638, MGC129613, P450RAI-2 , cytochrome P450 retinoid metabolizing protein, cytochrome P450, family 26, subfamily b, polypeptide 1, retinoic acid-metabolizing cytochrome
Protein Accession Number:
NP_063938.1
Protein Gi Number:
9845285
Ncbi Gene Id Number:
56603
Background Reference 1:
Bowles J, Knight D, Smith C, Wilhelm D, Richman J, Mamiya S, Yashiro K, Chawengsaksophak K, Wilson MJ, Rossant J, Hamada H, Koopman P. Retinoid signaling determines germ cell fate in mice. Science. 2006 Apr 28;312(5773):596-600. Epub 2006 Mar 30.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-447

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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