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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
CYP2A7 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

CYP2A7 Antibody

CATALOG NUMBER: 55-205

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Mouse, Rabbit
Immunogen:
This CYP2A7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-148 amino acids from the Central region of human CYP2A7.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
56 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CYP2A7
Additional Names:
Cytochrome P450 2A7, CYPIIA7, Cytochrome P450 IIA4, CYP2A7
Protein Accession Number:
P20853
Protein Gi Number:
215273959
Ncbi Gene Id Number:
1549
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CYP2A7 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum; its substrate has not yet been determined. CYP2A7, which produces two transcript variants, is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.
Background Reference 1:
Fukami, T., et al. Pharmacogenomics J. 6(6):401-412(2006) Nelson, D.R., et al. Pharmacogenetics 14(1):1-18(2004) Oscarson, M., et al. Hum. Mutat. 20(4):275-283(2002) Godoy, W., et al. Carcinogenesis 23(4):611-616(2002) Koskela, S., et al. Biochem. Pharmacol. 57(12):1407-1413(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-205

List Size:
400 ul

List Price:

$495.00

Datasheet
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