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Western blot analysis of extracts of various cell lines, using CYP3A4 antibody (18-699) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat ovary using CYP3A4 antibody (18-699) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using CYP3A4 antibody (18-699) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CYP3A4 antibody (18-699) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CYP3A4 antibody (18-699) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse liver using CYP3A4 antibody (18-699) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CYP3A4 antibody (18-699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

CYP3A4 Antibody

CATALOG NUMBER: 18-699

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A4 (NP_059488.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:20 - 1:50
IF: 1:20 - 1:100
Positive Control 1:
HT-29
Positive Control 2:
HepG2
Positive Control 3:
293T
Positive Control 4:
Jurkat
Positive Control 5:
SKOV3
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 80kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CYP3A4
Additional Names:
CYP3A4, cytochrome P450, family 3, subfamily A, polypeptide 4, HGNC:2637, CP33, CP34, CYP3A, CYP3A3, HLP, NF-25, P450C3, P450PCN1, P450-III, steroid inducible, subfamily IIIA (niphedipine oxidase), polypeptide 3, subfamily IIIA, glucocorticoid-inducible P450, nifedipine oxidase
Ncbi Gene Id Number:
1576
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-699

List Size:
100 uL

List Price:

$436.00

Datasheet
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