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45-448 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
45-448 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo

CYP3A4 Antibody

CATALOG NUMBER: 45-448

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KESRLEDTQKHR
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_059488.2; NP_001189784.1) and it is not expected to cross-react with 3A5, 3A7 ot 3A43.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CYP3A4
Additional Names:
CYP3A4, cytochrome P450, family 3, subfamily A, polypeptide 4 , HGNC:2637, CP33, CP34, CYP3A, CYP3A3, HLP, NF-25, P450C3, P450PCN1, P450-III, steroid inducible, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4, cytochrome P450, subfamily IIIA, polypeptide 4, glucocorticoid-inducible P450, nifedipine oxidase
Protein Accession Number:
NP_059488.2,NP_001189784.1
Protein Gi Number:
13435386
Ncbi Gene Id Number:
1576
Background Reference 1:
Choi MH, Skipper PL, Wishnok JS, Tannenbaum SR. Characterization of testosterone 11{beta}-hydroxylation catalyzed by human-liver microsomal cytochromes P450. Drug Metab Dispos. 2005 Mar 11;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-448

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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