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Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CYP3A43 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

CYP3A43 Antibody

CATALOG NUMBER: 63-328

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CYP3A43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-270 amino acids from the Central region of human CYP3A43.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:2000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CYP3A43
Additional Names:
Cytochrome P450 3A43, CYP3A43
Protein Accession Number:
Q9HB55
Protein Gi Number:
20137481
Ncbi Gene Id Number:
64816
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CYP3A43 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, the protein unknown whether the enzyme is also involved in xenobiotic metabolism.
Background Reference 1:
Domanski T.L., Finta C.Mol. Pharmacol. 59:386-392(2001)
Background Reference 2:
Westlind A., Malmebo S.Biochem. Biophys. Res. Commun. 281:1349-1355(2001)
Background Reference 3:
Cauffiez C., Lo-Guidice J.-M.Hum. Mutat. 23:101-101(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-328

List Size:
400 ul

List Price:

$495.00

Datasheet
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