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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
CYP5A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse kidney tissue lysates (35ug/lane).

CYP5A1 Antibody

CATALOG NUMBER: 56-215

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey
Immunogen:
This CYP5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-257 amino acids from the Central region of human CYP5A1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
61 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TBXAS1
Additional Names:
Thromboxane-A synthase, TXA synthase, TXS, Cytochrome P450 5A1, TBXAS1, CYP5, CYP5A1
Protein Accession Number:
P24557
Ncbi Gene Id Number:
6916
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the cytochrome P450superfamily of enzymes. The cytochrome P450 proteins aremonooxygenases which catalyze many reactions involved in drugmetabolism and synthesis of cholesterol, steroids and other lipids.However, this protein is considered a member of the cytochrome P450superfamily on the basis of sequence similarity rather thanfunctional similarity. This endoplasmic reticulum membrane proteincatalyzes the conversion of prostglandin H2 to thromboxane A2, apotent vasoconstrictor and inducer of platelet aggregation. Theenzyme plays a role in several pathophysiological processesincluding hemostasis, cardiovascular disease, and stroke.Alternatively spliced transcript variants encoding differentisoforms have been found for this gene.
Background Reference 1:
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Background Reference 2:
Wang, L.H., et al. Prostaglandins Other Lipid Mediat. 68-69, 409-422 (2002) :
Background Reference 3:
Tazawa, R., et al. Arch. Biochem. Biophys. 334(2):349-356(1996)
Background Reference 4:
Baek, S.J., et al. Gene 173(2):251-256(1996)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-215

List Size:
400 ul

List Price:

$495.00

Datasheet
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