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Western blot analysis of CYP7B1 Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with CYP7B1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CYP7B1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of CYP7B1 Antibody in Hela cell line lysates (35ug/lane)

CYP7B1 Antibody

CATALOG NUMBER: 63-643

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CYP7B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-281 amino acids from the Central region of human CYP7B1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CYP7B1
Additional Names:
25-hydroxycholesterol 7-alpha-hydroxylase, Cytochrome P450 7B1, Oxysterol 7-alpha-hydroxylase, CYP7B1
Protein Accession Number:
O75881
Ncbi Gene Id Number:
9420
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CYP7B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.
Background Reference 1:
Schwarz,M., et.al., Curr. Opin. Lipidol.(2), 113-118 (1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-643

List Size:
400 ul

List Price:

$495.00

Datasheet
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