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45-450 (0.1ug/ml) staining of Human Lungs (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-450 (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data was obtained using a previous batch.
45-450 (0.1ug/ml) staining of Human Lungs (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Cystatin B Antibody

CATALOG NUMBER: 45-450

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QTNKAKHDELTYF
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 16kDa band observed in Human Liver, Lung and Kidney (calculated MW of 11.1kDa according to NP_000091.1 ). The observed molecular weight corresponds to earlier findings in literature with with different antibodies (Lee et al, Clin Cancer Res. 2008 Feb 15;14(4):1080-9.; PMID: 18281540 ). Recommended concentration: 0.1-0.3ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CSTB
Additional Names:
CSTB, cystatin B (stefin B), PME, CST6, STFB, CPI-B, stefin B, liver thiol proteinase inhibitor, EPM1, cystatin B
Protein Accession Number:
NP_000091.1
Protein Gi Number:
4503117
Ncbi Gene Id Number:
1476
Background Reference 1:
Jerala R, Trstenjak M, Lenarcic B, Turk V. Cloning a synthetic gene for human stefin B and its expression in E. coli. FEBS Lett. 1988 Oct 24;239(1):41-4.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-450

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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