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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

CYTH4 Antibody

CATALOG NUMBER: 58-533

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CYTH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-319 amino acids from the C-terminal region of human CYTH4.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CYTH4
Additional Names:
Cytohesin-4, PH, SEC7 and coiled-coil domain-containing protein 4, CYTH4, CYT4, PSCD4
Protein Accession Number:
Q9UIA0
Protein Gi Number:
13124094
Ncbi Gene Id Number:
27128
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the PSCDfamily. Members of this family have identical structuralorganization that consists of an N-terminal coiled-coil motif, acentral Sec7 domain, and a C-terminal pleckstrin homology (PH)domain. The coiled-coil motif is involved in homodimerization, theSec7 domain contains guanine-nucleotide exchange protein (GEP)activity, and the PH domain interacts with phospholipids and isresponsible for association of PSCDs with membranes. Members ofthis family appear to mediate the regulation of protein sorting andmembrane trafficking. The encoded protein exhibits GEP activity invitro with both ARF1 and ARF5 but is inactive with ARF6. Thestructures of this gene and CYTH1 are very similar. [provided byRefSeq].
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Morishige, M., et al. Nat. Cell Biol. 10(1):85-92(2008)
Background Reference 3:
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Background Reference 4:
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-533

List Size:
400 ul

List Price:

$495.00

Datasheet
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