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45-452 (2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-452 (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-452 (2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Cytochrome B Reductase 1 Antibody

CATALOG NUMBER: 45-452

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: CRNLALDEAGQRSTM
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1µg/ml antibody staining. The detected bands were all successfully blocked by with the immunizing peptide. This antibody was successfully used in WB on Human in PMID: 24894955, 21973163 and 18830567.Immunohistochemistry:In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration: 2-5ug/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 2.5ug/ml.
Specificity:
This antibody is expected to recognise isoform 1 (NP_079119.3) only.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CYBRD1
Additional Names:
CYBRD1, DCYTB, FLJ23462, cytochrome b reductase 1, duodenal cytochrome b, FRRS3, ferric-chelate reductase 3
Protein Accession Number:
NP_079119.3
Protein Gi Number:
188536036
Ncbi Gene Id Number:
79901
Background Reference 1:
Atanasova BD, Li AC, Bjarnason I, Tzatchev KN, Simpson RJ. Duodenal ascorbate and ferric reductase in human iron deficiency. Am J Clin Nutr. 2005 Jan;81(1):130-3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-452

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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