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Western blot analysis of extracts of various cell lines, using Cytochrome c antibody (14-986) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of HeLa cells using Cytochrome c antibody (14-986) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Cytochrome c antibody (14-986) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Cytochrome c Antibody

CATALOG NUMBER: 14-986

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
SH-SY5Y
Positive Control 2:
293T
Positive Control 3:
BT-474
Positive Control 4:
22Rv1
Positive Control 5:
THP-1
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 14kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CYCS
Additional Names:
CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic
Ncbi Gene Id Number:
54205
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-986

List Size:
100 uL

List Price:

$436.00

Datasheet
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