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43-597 (0.3ug/ml) staining of A549 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-597 (0.3ug/ml) staining of A549 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

cytochrome P450 1A1 Antibody

CATALOG NUMBER: 43-597

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CQEKQLDENANVQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 85-90kDa band observed in lysates of cell lines A549 and MCF7 (calculated MW of 58.2kDa according to NP_000490.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is not expected to cross-react with the similar P450 1A2
Predicted Molecular Weight:
Approx 85-90kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
cytochrome P450 1A1
Additional Names:
CYP1A1, cytochrome P450, family 1, subfamily A, polypeptide 1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX, CYPIA1, OTTHUMP00000183469, aryl hydrocarbon hydroxylase, cytochrome P1-450, dioxin-inducible, cytochrome P450 1A1, cytochrome P450 form 6, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1, cytochrome P450-C, cytochrome P450-P1, flavoprotein-linked monooxygenase, xenobiotic monooxygenase
Protein Accession Number:
NP_000490.1
Protein Gi Number:
4503199
Ncbi Gene Id Number:
1543
Background Reference 1:
Sulem P, Gudbjartsson DF, Geller F, Prokopenko I, Feenstra B, Aben KK, Franke B, den Heijer M, Kovacs P, Stumvoll M, Mägi R, Yanek LR, Becker LC, Boyd HA, Stacey SN, Walters GB, Jonasdottir A, Thorleifsson G, Holm H, Gudjonsson SA, Rafnar T, BjörnsdottirSequence variants at CYP1A1-CYP1A2 and AHR associate with coffee consumption.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-597

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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