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43-484 (0.1ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-484 (0.1ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

cytokeratin 19 Antibody

CATALOG NUMBER: 43-484

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-EGQEDHYNNLSASK
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 43kDa band observed in Human cancer lysates of Breast, Pancreas and Colon (calculated MW of 44.1kDa according to NP_002267.2). Recommended concentration: 0.03-0.1ug/ml.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA both as a capturer and as reporter antibody in combination with EB12359
Predicted Molecular Weight:
Approx 43kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
cytokeratin 19
Additional Names:
KRT19, keratin 19, CK19, K19, K1CS, 40-kDa keratin intermediate filament, CK-19, cytokeratin 19, cytokeratin-19, keratin, type I cytoskeletal 19, keratin, type I, 40-kd, keratin-19
Protein Accession Number:
NP_002267.2
Protein Gi Number:
24234699
Ncbi Gene Id Number:
3880
Background Reference 1:
Alix-Panabières C, Vendrell JP, Slijper M, Pellé O, Barbotte E, Mercier G, Jacot W, Fabbro M, Pantel K.Full-length cytokeratin-19 is released by human tumor cells: a potential role in metastatic progression of breast cancer.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-484

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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