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Western blot analysis of various lysates using Cytokeratin 19 (CK19) Rabbit mAb at 1:500 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): HeLa | Exposure time: 0.5s.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Cytokeratin 19 (CK19) Rabbit mAb at a dilution of 1:2500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Cytokeratin 19 (CK19) Rabbit mAb at a dilution of 1:2500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Cytokeratin 19 (CK19) Rabbit mAb at a dilution of 1:2500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of MCF7 cells using Cytokeratin 19 (CK19) Rabbit mAb followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (Red). The cells were counterstained with α-Tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of paraffin-embedded Human colon cancer tissue using Cytokeratin 19 (CK19) Rabbit mAb followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Western blot analysis of various lysates using Cytokeratin 19 (CK19) Rabbit mAb at 1:500 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): HeLa | Exposure time: 0.5s.

Cytokeratin 19 (CK19) Rabbit Recombinant Antibody (81-324)

CATALOG NUMBER: 81-324

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, IF/ICC, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, IF/ICC, IHC-P, WB
Application Note:
WB,1:500 - 1:3000, IHC-P,1:200 - 1:800, IF/ICC,1:100 - 1:400, IF-P,1:100 - 1:400, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
BxPC-3, MCF7
Predicted Molecular Weight:
44 kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KRT19
Additional Names:
K19; KRT19; K1CS; Cytokeratin 19 (CK19)
Protein Accession Number:
P08727
Ncbi Gene Id Number:
3880
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-324

List Size:
100 uL

List Price:

$540.00

Datasheet
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