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43-167 (0.03ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-167 (0.03ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

cytokeratin 20 Antibody

CATALOG NUMBER: 43-167

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-ETNAPRAGRDYS
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 49kDa band observed in Human Colon and Ileum lysates (calculated MW of 48.5kDa according to NP_061883.1). Recommended concentration: 0.01-0.03ug/ml.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA as a reporter in combination with capture antibody EB11746.
Predicted Molecular Weight:
Approx 49kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
cytokeratin 20
Additional Names:
KRT20, keratin 20, CK-20, CK20, K20, KRT21, MGC35423, OTTHUMP00000164518, cytokeratin 20, cytokeratin-20, keratin, type I cytoskeletal 20, keratin-20, protein IT
Protein Accession Number:
NP_061883.1
Protein Gi Number:
27894337
Ncbi Gene Id Number:
54474
Background Reference 1:
Magnusson K, de Wit M, Brennan DJ, Johnson LB, McGee SF, Lundberg E, Naicker K, Klinger R, Kampf C, Asplund A, Wester K, Gry M, Bjartell A, Gallagher WM, Rexhepaj E, Kilpinen S, Kallioniemi OP, Belt E, Goos J, Meijer G, Birgisson H, Glimelius B, BorrebaecSATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinomas.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-167

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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