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IHC analysis of FFPE human colon carcinoma with Cytokeratin 8/18 antibody (clone KRT8.18/1346). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC analysis of FFPE human prostate carcinoma with Cytokeratin 8/18 antibody (clone KRT8.18/1346). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC analysis of FFPE human colon carcinoma with Cytokeratin 8/18 antibody (clone KRT8.18/1346). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Cytokeratin 8/18 Antibody [KRT8.18/1346]

CATALOG NUMBER: 33-683

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A cytoskeleton preparation from HeLa cells was used as the immunogen for the Cytokeratin 8/18 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
KRT18
Additional Names:
Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18
Ncbi Gene Id Number:
3875
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-683

List Size:
100 ug

List Price:

$626.00

Datasheet
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