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Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 8 antibody (SPM538).
Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 8 antibody (SPM538).

Cytokeratin 8 Antibody [SPM538]

CATALOG NUMBER: 34-125

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC
Host Species:
Mouse
Species Reactivity:
Human, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Rat, Zebrafish
Immunogen:
A cytoskeleton preparation containing keratin 8 was used as the immunogen for this Cytokeratin 8 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the Cytokeratin 8 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
KRT8
Additional Names:
KRT8, CARD2, CK8, CYK8, K2C8, K8, KO, CK-8
Ncbi Gene Id Number:
3856
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-125

List Size:
100 ug

List Price:

$626.00

Datasheet
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