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Western blot analysis using CK monoclonal antibody against truncated CK recombinant protein.
Immunohistochemical analysis of paraffin - embedded human colon adenocarcinoma (top) showing cytoplasma / membrane location using CK antibody with DAB staining. Imunofluorescense staining of methanol - fixed HepG - 2 cells (bottom) showing cytoplasm localization.
Western blot analysis using CK monoclonal antibody against truncated CK recombinant protein.

Cytokeratin Antibody [7H8C4]

CATALOG NUMBER: 32-143

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant CK expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Cytokeratin(Pan) monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KRT5
Additional Names:
58 kDa cytokeratin, CK-5, K5, CK5, DDD, DDD1, EBS2, KRT5A
Protein Accession Number:
P13647
Protein Gi Number:
143811411
Ncbi Gene Id Number:
3852
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.
Background Reference 1:
Scope A. Schwendenwein I. Frommlet F. Vet Rec. 2006, Dec 16, 159(25): 839-43.
Background Reference 2:
Somjen D. Katzburg S. Posner GH. et al. J Cell Biochem. 2007, Apr 15, 100(6): 1406-14.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-143

List Size:
0.1 mL

List Price:

$432.00

Datasheet
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