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Western blot analysis using CK5 monoclonal antibody against truncated CK5 - Trx - His recombinant protein
Immunohistochemical analysis of paraffin - embedded human normal esophagus epithelium (top) and human human normal salivary gland basal cell (bottom) showing cytoplasma / membrane location using CK5 antibody with DAB staining.
Western blot analysis using CK5 monoclonal antibody against truncated CK5 - Trx - His recombinant protein

Cytokeratin5 Antibody [3E2F1]

CATALOG NUMBER: 32-142

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant CK5-Trx-His expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Cytokeratin 5 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KRT5
Additional Names:
58 kDa cytokeratin, CK-5, K5, CK5, DDD, DDD1, EBS2, KRT5A
Protein Accession Number:
P13647
Protein Gi Number:
143811411
Ncbi Gene Id Number:
3852
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.
Background Reference 1:
Canedo-Patzi AM. Leon-Bojorge B. de Ortiz-Hidalgo C. Gac Med Mex. 2006, Jan-Feb, 142(1):59-66. Spanish.
Background Reference 2:
Leibl S. Moinfar F. Am J Surg Pathol. 2006, Apr, 30(4):450-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-142

List Size:
0.1 mL

List Price:

$495.00

Datasheet
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