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45-455 (0.1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-455 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-455 (3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-455 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-455 (0.1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

D54 Antibody

CATALOG NUMBER: 45-455

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-SGDKPLSDPAP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Breast Cancer and Human Brain lysates (calculated MW of 24.9kDa according to NP_955392.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. See also our D52 and D53 products.Immunohistochemistry:Paraffin embedded Human Spleen, Tonsil and Brain (Cortex). Recommended concentration: 3.75ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificity:
This antibody is expected to recognise all reported isoforms (NP_955392.1; NP_955393.1; NP_955394.1; NP_955395.1; NP_003279.2; NP_955391.1).
Positive Control 1:
Cat. No. 1323 - Human Breast Tumor Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TPD52L2
Additional Names:
TPD52L2, D54, tumor protein D52-like 2 , DKFZp686A1765, hD54, HCCR-binding protein 2
Protein Accession Number:
NP_955392.1,NP_955393.1,NP_955394.1,NP_955395.1,NP_003279.2,NP_955391.1
Protein Gi Number:
40805864
Ncbi Gene Id Number:
7165
Background Reference 1:
Reynolds JL, Mahajan SD, Sykes D, Nair MP. Heroin-Induces Differential Protein Expression by Normal Human Astrocytes (NHA). Am J Infect Dis. 2006;2(2):49-57.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-455

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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