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Western blot analysis of DAAM1 using rabbit polyclonal DAAM1 Antibody(Human N-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAAM1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of DAAM1 using rabbit polyclonal DAAM1 Antibody(Human N-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAAM1 gene (Lane 2).

DAAM1 Antibody

CATALOG NUMBER: 61-449

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This DAAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human DAAM1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
123 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DAAM1
Additional Names:
Disheveled-associated activator of morphogenesis 1, DAAM1, KIAA0666
Protein Accession Number:
Q9Y4D1
Protein Gi Number:
34098767
Ncbi Gene Id Number:
23002
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton. Recent evidence suggests a role for the Formin homology (FH) proteins in these processes. DAAM1 contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by DAAM1, which is thought to function as a scaffolding protein.
Background Reference 1:
Liu,W., Proc. Natl. Acad. Sci. U.S.A. 105 (1), 210-215 (2008)
Background Reference 2:
Yamashita,M.,Genes Cells 12 (11), 1255-1265 (2007)
Background Reference 3:
Lu,J., J. Mol. Biol. 369 (5), 1258-1269 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-449

List Size:
400 ul

List Price:

$495.00

Datasheet
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