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Western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DAAM1 antibody detected the DAAM1 protein (arrow).
Western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DAAM1 antibody detected the DAAM1 protein (arrow).
DAAM1-T361 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DAAM1 antibody detected the DAAM1 protein (arrow).

DAAM1-T361 Antibody

CATALOG NUMBER: 61-929

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Immunogen:
This DAAM1-T361 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the N-terminal region of human DAAM1-T361.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
123 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DAAM1
Additional Names:
Disheveled-associated activator of morphogenesis 1, DAAM1, KIAA0666
Protein Accession Number:
Q9Y4D1
Protein Gi Number:
34098767
Ncbi Gene Id Number:
23002
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Functions of the cell cortex, including motility,adhesion, and cytokinesis, are mediated by the reorganization ofthe actin cytoskeleton and recent evidence suggests a role for theFormin homology (FH) proteins in these processes. The proteinencoded by this gene contains FH domains and belongs to a novel FHprotein subfamily implicated in cell polarity. Wnt/Fz signalingactivates the small GTPase Rho, a key regulator of cytoskeletonarchitecture, to control cell polarity and movement duringdevelopment. Activation requires Dvl-Rho complex formation, anassembly mediated by this gene product, which is thought tofunction as a scaffolding protein. Evidence of alternative splicinghas been observed for this gene but the full-length nature of thesevariants has not been determined.
Background Reference 1:
Higashi, T., et al. J. Biol. Chem. 283(13):8746-8755(2008)
Background Reference 2:
Liu, W., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):210-215(2008)
Background Reference 3:
Yamashita, M., et al. Genes Cells 12(11):1255-1265(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-929

List Size:
400 ul

List Price:

$495.00

Datasheet
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