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Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.
Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.
Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Trial Size

DAD1 Antibody

CATALOG NUMBER: 3313

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Pig: (100%), Bovine: (100%), Chicken: (85%)
Immunogen:
DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.
The immunogen is located within the last 50 amino acids of DAD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 17-011 - Hep G2 Cell Slide
Purification:
DAD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DAD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DAD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DAD1
Additional Names:
DAD1 Antibody: OST2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Defender against cell death 1, Oligosaccharyl transferase subunit DAD1
Protein Accession Number:
AAH09798
Protein Gi Number:
14602573
Ncbi Gene Id Number:
1603
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DAD1 Antibody: Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
Background Reference 1:
Nakashima T, Sekiguchi T, Kuraoka A, et al. Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. Mol. Cell Biol. 1993; 13:6367-74.
Background Reference 2:
Stellar H. Mechanisms and genes of cellular suicide. Science 1995; 267:1445-9.
Background Reference 3:
Gilmore R and Kelleher DJ. DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase. Proc. Natl. Acad. Sci. USA 1997; 94:4994-9.
Background Reference 4:
Sanjay A, Fu J, and Kreibich G. DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex. J. Biol. Chem. 1998; 273:26094-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3313

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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