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Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 μg/ml.
Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 μg/ml.
Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 μg/ml.
Western blot analysis of DAF1 expression in HeLa cell lysate with DAF1 antibody at (A) 1 and (B) 2 μg/ml.
Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 μg/ml.
Trial Size

DAF Antibody

CATALOG NUMBER: 7937

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF.
The immunogen is located within amino acids 360 - 410 of DAF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Positive Control 3:
Cat. No. 1201 – HeLa Cell Lysate
Predicted Molecular Weight:
Predicted: 48 kDa
Observed: 49 kDa
Purification:
DAF antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DAF antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DAF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CD55
Additional Names:
Decay-accelerating factor, CD55 molecule decay accelerating factor for complement (Cromer blood group), CD55, CR, TC, CROM
Protein Accession Number:
NP_001108224
Protein Gi Number:
168693643
Ncbi Gene Id Number:
1604
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The decay-accelerating factor (DAF), also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1). DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2). Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3).
Background Reference 1:
Nicholson-Weller A, March JP, Rosen CE, et al. Surface membrane expression by human blood leukocytes and platelts of decay-accelerating factor, a regulatory protein of the complement system. Blood 1985; 65:1237-44.
Background Reference 2:
Seya T and Atkinson JP. Functional properties of membrane cofactor protein of complement. Biochem. J. 1989; 64:581-8.
Background Reference 3:
Storry JR, Reid ME, and Yazer MH. The Cromer blood group system: a review. Immunohematology 2010; 109-18.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7937

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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