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Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
DAG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.

DAG1 Antibody

CATALOG NUMBER: 57-475

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit, Pig
Immunogen:
This DAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 718-747 amino acids from the C-terminal region of human DAG1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000-1:2000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
97 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DAG1
Additional Names:
Dystroglycan, Dystrophin-associated glycoprotein 1, Alpha-dystroglycan, Alpha-DG, Beta-dystroglycan, Beta-DG, DAG1
Protein Accession Number:
Q14118
Protein Gi Number:
229462879
Ncbi Gene Id Number:
1605
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Dystroglycan is a laminin binding component of thedystrophin-glycoprotein complex which provides a linkage betweenthe subsarcolemmal cytoskeleton and the extracellular matrix.Dystroglycan 1 is a candidate gene for the site of the mutation inautosomal recessive muscular dystrophies. The dramatic reduction ofdystroglycan 1 in Duchenne muscular dystrophy leads to a loss oflinkage between the sarcolemma and extracellular matrix, renderingmuscle fibers more susceptible to necrosis. Dystroglycan alsofunctions as dual receptor for agrin and laminin-2 in the Schwanncell membrane. The muscle and nonmuscle isoforms of dystroglycandiffer by carbohydrate moieties but not protein sequence.Alternative splicing results in multiple transcript variants allencoding the same protein.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Nilsson, J., et al. Glycobiology 20(9):1160-1169(2010)
Background Reference 3:
Lara-Chacon, B., et al. J. Cell. Biochem. 110(3):706-717(2010)
Background Reference 4:
Sgambato, A., et al. Pathology 42(3):248-254(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-475

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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