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46-829 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-829 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-829 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

DAGLA Antibody

CATALOG NUMBER: 46-829

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-PAKQDELVISAR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 125kDa band observed in Human Liver lysates (calculated MW of 115kDa according to NP_006124.1). Recommended concentration: 0.3-1ug/ml. Some minor background is detected and is blocked by the immunizing peptide. Preliminary testing was unsuccessful on Mouse for this particular batch.Immunohistochemistry:Paraffin embedded Human Breast, Pancreas and Spleen. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DAGLA
Additional Names:
DAGLA, diacylglycerol lipase, alpha, C11orf11, DAGL(ALPHA), DAGLALPHA, NSDDR, diacylglycerol lipase alpha, neural stem cell-derived dendrite regulator, KIAA0659
Protein Accession Number:
NP_006124.1
Protein Gi Number:
27262632
Ncbi Gene Id Number:
747
Background Reference 1:
Bisogno T, Howell F, Williams G, Minassi A, Cascio MG, Ligresti A, Matias I, Schiano-Moriello A, Paul P, Williams EJ, Gangadharan U, Hobbs C, Di Marzo V, Doherty P. Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain. J Cell Biol. 2003 Nov 10;163(3):463-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-829

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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