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Western blot analysis of DAP in human small intestine tissue lysate with DAP antibody at 1 μg/mL.
Immunohistochemistry of DAP in human small intestine tissue with DAP antibody at 5 μg/ml.
Immunofluorescence of DAP in human small intestine tissue with DAP antibody at 20 μg/ml.
Western blot analysis of DAP in human small intestine tissue lysate with DAP antibody at 1 μg/mL.
Trial Size

DAP Antibody

CATALOG NUMBER: 7389

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (89%)
Immunogen:
DAP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DAP.
The immunogen is located within the last 50 amino acids of DAP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
DAP antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 11 kDa
Observed: 11 kDa
Purification:
DAP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
DAP
Additional Names:
DAP Antibody: DAP1, Death-associated protein 1, DAP-1
Protein Accession Number:
NP_004385
Protein Gi Number:
4758120
Ncbi Gene Id Number:
1611
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).
Background Reference 1:
Deiss LP, Feinstein E, Berissi H, et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev. 1995; 9:15-30.
Background Reference 2:
Koren I, Reem E, and Kimchi A. DAP1, a novel substrate of mTOR, negatively regulates autophagy. Curr. Biol. 2010; 20:1093-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7389

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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