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Western blot analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody at 1 μg/mL.
Immunohistochemistry of DAPK2 in mouse spleen cells with DAPK2 antibody at 2 μg/mL.
Western blot analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody at 1 μg/mL.
Trial Size

DAPK2 Antibody

CATALOG NUMBER: 2323

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
DAPK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2.
This antigenic peptide is identical to the corresponding amino acids of mouse origin (1,2).
The immunogen is located within the last 50 amino acids of DAPK2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
DAPK2 antibody can be used for detection of DAPK2 by Western blot at 1 μg/mL. An approximately 42 kDa band can be detected. DAPK2 has no cross responses to DAPK1. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
DAPK2 has no cross responses to DAPK1.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Positive Control 3:
Cat. No. 1465 - Rat Kidney Tissue Lysate
Predicted Molecular Weight:
42 kDa
Purification:
DAPK2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DAPK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
DAPK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DAPK2
Additional Names:
DAPK2 Antibody: DRP1, DRP-1, Death-associated protein kinase 2, DAP-kinase-related protein 1, DAP kinase 2
Protein Accession Number:
BAA88063
Protein Gi Number:
6521210
Ncbi Gene Id Number:
23604
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DAPK2 Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNγ induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.
Background Reference 1:
Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ, Jenkins NA, Akira S. Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity. Oncogene 1999;18(23):3471-80
Background Reference 2:
Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A. Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis. Mol Cell Biol 2000;20(3):1044-54 (WD0101)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2323

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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