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Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.
Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.

DARPP-32 Antibody

CATALOG NUMBER: 50-201

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Bovine, Chicken, Dog, Frog, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Bovine, Chicken, Dog, Frog, Human, Mouse, Rat, Zebrafish
Immunogen:
Peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, human, mouse, rat, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Predicted Molecular Weight:
32
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
DARPP-32 antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
Ncbi Official Symbol:
Ppp1r1b
Protein Accession Number:
Q6J4I0
Protein Gi Number:
81884861
Ncbi Gene Id Number:
360616
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important rolesin the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).
Background Reference 1:
Bibb JA, Snyder GL, Nishi A, Yan Z, Meijer L, Fienberg AA, Tsai LH, Kwon YT, Girault JA, Czernik AJ, Huganir RL, Hemmings HC, Jr., Nairn AC, Greengard P (1999) Phosphorylation of DARPP-32 by cdk5 modulates dopamine signalling in neurons. Nature (London) 402:669-671.
Background Reference 2:
Fienberg, A.A., Hiroi, N., Mermelstein, P.G., Song, W., Snyder, G.L., Nishi, A., Cheramy, A. O'Callaghan, J.P., Miller, D.B., Cole, D.G., Corbett, R., Haile, C.N., Cooper, D,C., Onn, S.P., Grace, A.A., Ouimet, C.C., White, F.G., Hyman, S.E., Surmeier, D.G., Girault, J., Nestler, E.J. and Greengard, P. (1998) DARPP-32: regulator of the efficacy of dopaminergic neurotransmission. Science 281:838-842.
Background Reference 3:
Lindskog M, Svenningsson P, Pozzi L, Kim Y, Fienberg AA, Bibb JA, Fredholm BB, Nairn AC, Greengard P, Fisone G (2002) Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine. Nature (London) 418:774-778

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

50-201

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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