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Western blot of rat caudate lysate showing phosphospecific immunolabeling of the ~32k DARPP-32 protein phosphorylated at Thr75.
Western blot of rat caudate lysate showing phosphospecific immunolabeling of the ~32k DARPP-32 protein phosphorylated at Thr75.

DARPP 32 (phospho Thr75) Antibody

CATALOG NUMBER: XPS-1005

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
DARPP-32 (Thr75) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr75 of DARPP-32. The sequence of the immunogen is identical in human and mouse.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Rabbit anti-DARPP-32 (Thr75) recognizes human and mouse forms of the protein. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
DARPP 32 antibody is specific for the ~34k DARPP-32 phosphorylated at Thr75.
Predicted Molecular Weight:
32
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
Ncbi Official Symbol:
Ppp1r1b
Protein Accession Number:
Q6J4I0
Protein Gi Number:
81884861
Ncbi Gene Id Number:
360616
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
Background Reference 1:
Svenningsson, P., Tzavara, E.T., Carruthers, R., Rachleff, I., Wattler, S., Nehls, M., McKinzie, D.L., Fienberg, A.A., Nomikos, G.G.and Greengard, P., "Diverse psychotomimetics act through a common signaling pathway," Science, 302 (2003) 1412 - 1415.
Background Reference 2:
Lindskog, M., Svenningsson, P., Pozzi, L., Kim, Y., Fienberg, A.A., Bibb, J.A.., Fredholm, B.B., Nairn, A.C., Greengard, P. and Fisone, G., "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine, Nature (London), 418 (2002) 774 - 778.
Background Reference 3:
Maldve, R.E., Zhang, T.A., Ferrani-Kile, K., Schreiber, S.S., Lippmann, M.J., Snyder, G.L., Feinberg, A.A., Leslie, S.W., Gonzales, R.A., and Morrisett, R.A., "DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens," Nature Neurosci. 5 (2002) 641 - 648.
Background Reference 4:
Greengard, P., "The neurobiology of slow synaptic transmission," Science 294 (2001) 1024 -1030.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1005

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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