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45-461 (0.5ug/ml) staining of human testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-461 (0.5ug/ml) staining of human testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

DAX1 Antibody

CATALOG NUMBER: 45-461

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CGEDHPQQGSTLY
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50-55kDa band observed in human ovary and human testis lysates (calculated MW of 51.7kDa according to NP_000466.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NR0B1
Additional Names:
NR0B1, DAX1, nuclear receptor subfamily 0, group B, member 1, AHC, AHCH, AHX, DAX-1, DSS, GTD, HHG, NROB1 , dosage-sensitive sex reversal, gonadotropin deficiency, nuclear hormone receptor
Protein Accession Number:
NP_000466.2
Protein Gi Number:
5016090
Ncbi Gene Id Number:
190
Background Reference 1:
Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH. A protein interaction network for pluripotency of embryonic stem cells. Nature. 2006 Nov 16;444(7117):364-8. Epub 2006 Nov 8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-461

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

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