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Western blot analysis in CEM cell line lysates (35ug/lane).
DAZ3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in CEM cell line lysates (35ug/lane).

DAZ3 Antibody

CATALOG NUMBER: 56-335

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DAZ3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human DAZ3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
55 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DAZ3
Additional Names:
Deleted in azoospermia protein 3, DAZ3
Protein Accession Number:
Q9NR90
Protein Gi Number:
44887842
Ncbi Gene Id Number:
57054
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the DAZ gene family and is acandidate for the human Y-chromosomal azoospermia factor (AZF). Itsexpression is restricted to premeiotic germ cells, particularly inspermatogonia. It encodes an RNA-binding protein that is importantfor spermatogenesis. Four copies of this gene are found onchromosome Y within palindromic duplications; one pair of genes ispart of the P2 palindrome and the second pair is part of the P1palindrome. Each gene contains a 2.4 kb repeat including a 72-bpexon, called the DAZ repeat; the number of DAZ repeats is variableand there are several variations in the sequence of the DAZ repeat.Each copy of the gene also contains a 10.8 kb region that may beamplified; this region includes five exons that encode an RNArecognition motif (RRM) domain. This gene contains one copy of the10.8 kb repeat.
Background Reference 1:
Kim, B., et al. Hum. Reprod. 24(6):1507-1515(2009)
Background Reference 2:
Lardone, M.C., et al. Fertil. Steril. 88(5):1318-1326(2007)
Background Reference 3:
A, Z.C., et al. Yi Chuan 28(9):1057-1060(2006)
Background Reference 4:
Kuo, P.L., et al. Fertil. Steril. 81(4):1034-1040(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-335

List Size:
400 ul

List Price:

$495.00

Datasheet
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